C-Reactive Protein (CRP), also known as PTX1, and pentraxin-related, is a sensitive biomarker for the early diagnostics and observation of curative effect of the liver diseases and malnutrition. Because it is mainly synthesized by hepatic cells, and its serum levels decreased significantly under the condition of liver injury or malnutrition.

Relatively high levels of hs-CRP in otherwise healthy individuals have been found to be predictive of an increased risk of a future heart attack, stroke, sudden cardiac death, and/or peripheral arterial disease, even when cholesterol levels are within an acceptable range.

People with higher hs-CRP values have the highest risk of cardiovascular disease and those with lower values have less risk. Specifically, individuals who have hs-CRP results at the high end of the normal range have 1.5 to 4 times the risk of having a heart attack as those with hs-CRP values at the low end of the normal range.

The American Heart Association and U.S. Centers for Disease Control and Prevention have defined risk groups as follows: Low risk: less than 1.0 mg/L; Average risk: 1.0 to 3.0 mg/L; High risk: above 3.0 mg/L

IMD developed β2-MG PETIA kit uses its unique antibodies and antigens, with accuracy and specificity fully validated using international standard.

This assay is a turbidimetric immunoassay for the quantitative measurement of CRP in human serum and plasma.  A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with CRP antibodies, is added into the cuvette and mixed. The presence of CRP in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of CRP in unknown samples can be interpolated from a reference curve using the standards provided.

Assay procedures may vary depending on the automated chemistry analyzer to be used. A general example of assay procedures is stated as follow:

1. Dispense 300µl of R1 into a clean cuvette

2. Add 3µl of sample and incubate at 37˚C for 5 minutes

3. Further add 100µl of R2

4. Read change of absorbance at Main Wavelength 570 nm and Sub Wavelength 800 nm for 8 minutes after the addition of R2

5. Calculate the concentration of CRP in unknown sample by interpolation from a reference curve using the standards provided

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