INTRODUCTION
C-Reactive Protein (CRP) is an annular pentameric protein of hepatic origin. C-reactive protein is accepted in clinical use as a major, although rather non-specific, marker of inflammation. In generally healthy people, CRP levels are usually less than 5 mg/L. In pathology, CRP concentration has an eno In generally healthy individuals, the circulating concentration of CRP ranges between 0.8 mg/L to 2.0 mg/L. CRP concentrations between 2 and 10 mg/L are considered as chronic inflammation, between 10-25 mg/L is suggestive of viral infection, whereas the highest levels of CRP (above 30 mg/L) are observed in bacterial infection, such as septic arthritis, meningitis and pneumonia. CRP level falls rapidly after successful antibiotic treatment, and monitoring CRP levels can be used to monitor the therapeutic outcomes. Additionally circulating CRP can be used for stratification of cardiovascular risk (see Cat# 51121)
Full range CRP PETIA kit developed by IMD can accurately measure CRP with an extremely wide range of concentration (0.1 mg-300 mg/L), and therefore can be used for assessment of both chronic inflammation and acute viral/bacterial infection. The assay is validated with international standard.
PRINCIPLE OF THE ASSAY
This assay is a turbidimetric immunoassay for the quantitative measurement of CRP in human serum and plasma. A standard or sample is added into a cuvette and mixed with the reaction buffer R1. After a short incubation, the test reagent R2, which is a suspension of microparticles coated with CRP antibodies, is added into the cuvette and mixed. The presence of CRP in the standard or sample causes the immune-particles to aggregate. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The concentration of CRP in unknown samples can be interpolated from a reference curve using the standards provided.
Assay Range: 0.1 – 300 mg/L
ASSAY PROCEDURE
Assay procedures may vary depending on the automated chemistry analyzer to be used. A general example of assay procedures is stated as follow:
1. Dispense 300µl of R1 into a clean cuvette
2. Add 2µl of sample and incubate at 37˚C for 5 minutes
3. Further add 100µl of R2
4. Read change of absorbance at Main Wavelength 570 nm and Sub Wavelength 800 nm for 8 minutes after the addition of R2
5. Calculate the concentration of CRP in unknown sample by interpolation from a reference curve using the standards provided
PACKING SPECIFICATIONS
Cat. No. Size Approximately tests 51120-05 R1: 15ml, R2: 5ml 100 51120-10 R1: 30ml, R2: 10ml 200 51120-20 R1: 60ml, R2: 20ml 400 51120-50 R1: 150ml, R2: 50ml 1000 51120-100 R1: 300ml, R2: 100ml 2000 For bulk order, please enquire
OTHER MATERIALS REQUIRED
Product Catalog Number Format Method Calibrator 51120-S1 5 x 1ml Control 51120-C1 2 x 1ml
Link to instructions and details (pdf)
