Human Chromodomain-helicase-DNA-binding Protein 4 (Mi2-beta)

(Cat. No.: 41A190-s10)

Introduction:

The Mi-2 protein is the myositis specific antigen, which is over-regulated during muscle regeneration in DM (dermatomyositis) patients.

Description:

Expressed in insect Sf9 cells with total 1936 AA. Mw: 221.0 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted). Recombinant antigen for research use or manufacturing only.

Immunological Function:

As an autoantigen, Mi-2beta binds with IgG-type human auto-antibodies.

Amino Acid Sequence:

MSYYHHHHHHDYDIPTTENLYFQGAASGLGSPSPCSAGSEEE DMDALLNNSLPPPHPENEEDPEEDLSETETPKLKKKKKPKKPR DPKIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDS EGSDYTPGKKKKKKLGPKKEKKSKSKRKEEEEEEDDDDDSKEP KSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQFVRPLIAA KNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAA VAVVESMVTATEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKG SPRVPDAKKPKPKKVAPLKIKLGGFGSKRKRSSSEDDDLDVESD FDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVDG YETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEG KWSCPHCEKEGIQWEAKEDNSEGEEILEEVGGDLEEEDDHHM EFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEIPNGEWLCPRC TCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLE GRPERQFFVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRK NDMDEPPSGDFGGDEEKSRKRKNKDPKFAEMEERFYRYGIKP EWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEI QDYDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPET PTVDPTVKYERQPEYLDATGGTLHPYQMEGLNWLRFSWAQGT DTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAPLSTIIN WEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGG KKASRMKKEASVKFHVLLTSYELITIDMAILGSIDWACLIVDEA HRLKNNQSKFFRVLNGYSLQHKLLLTGTPLQNNLEELFHLLNF LTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKAD VFKNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGN QVSLLNVVMDLKKCCNHPYLFPVAAMEAPKMPNGMYDGSALI RASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLEDFLEHE

GYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGI NLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVT RASVEERITQVAKKKMMLTHLVVRPGLGSKTGSMSKQELDDIL KFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQ DETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQ EESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYND GSQEDRDWQDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSR KGLRNDKDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRYG MPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADG AETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWS MPELAEVEENKKMSQPGSPSPKTPTPSTPGDTQPNTPAPVPPA EDGIKIEENSLKEEESIEGEKEVKSTAPETAIECTQAPAPASEDE KVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKS AIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKN IKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWHRRH DYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFLE IKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALN TRFAEVECLAESHQHLSKESMAGNKPANAVLHKVLKQLEELLS DMKADVTRLPATIARIPPVAVRLQMSERNILSRLANRAPEPTPQ QVAQQQ

Applications:

Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

Formulation:

Liquid in storage buffer (50mM Tris, 300- 500mM NaCl, 10% Glycerol, Protease inhibitor， pH8.0).

Storage:

Store at –80°C. Avoid repeated freezing/thawing cycles.

Quality Control Test:

BCA to determine quantity of the protein. SDS PAGE to determine purity of the protein. Immunodot analysis to determine functionality of protein.

SDS-PAGE Gel