Human Sp100
(Cat. No.: 41A162)
| Origin: | Recombinant | Cat. No.: | 41A162 |
| Concentration: | 2.97 mg/mL | Size: | 0.1 mg |
| Source: | Spodopterafrugiperda Sf9 | Purity: | >90% |
| Other names: | Species: | Human |
Introduction:
Together with PML, Sp100 is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Autoantibody against Sp100 are suggested as a significant role in the diagnosis of primary biliary cirrhosis.
Description:
Expressed in insect Sf9 cells with total 903 AA. Mw:100.04KDa(calculated). N-terminal 6xHis-tag and TEV cleavage site, extra 25 AA (highlighted). Recombinant antigen for research use or manufacturing only.
Immunological Function:
As an autoantigen, Sp100 binds with IgG-type human auto-antibodies.
Amino Acid Sequence:
MSYYHHHHHHDYDIPTTENLYFQGAAGGGGDLST RRLNECISPVANEMNHLPAHSHDLQRMFTEDQGV DDRLLYDIVFKHFKRNKVEISNAIKKTFPFLEGLR DRDLITNKMFEDSQDSCRNLVPVQRVVYNVLSEL EKTFNLPVLEALFSDVNMQEYPDLIHIYKGFENVI HDKLPLQESEEEEREERSGLQLSLEQGTGENSFRS LTWPPSGSPSHAGTTPPENGLSEHPCETEQINAKR KDTTSDKDDSLGSQQTNEQCAQKAEPTESCEQIAV QVNNGDAGREMPCPLPCDEESPEAELHNHGIQIN SCSVRLVDIKKEKPFSNSKVECQAQARTHHNQASD IIVISSEDSEGSTDVDEPLEVFISAPRSEPVINNDNP LESNDEKEGQEATCSRPQIVPEPMDFRKLSTFRES FKKRVIGQDHDFSESSEEEAPAEASSGALRSKHGEKAPMTSRSTSTWRIPSRKRRFSSSDFSDLSNGEEL QETCSSSLRRGSGSQPQEPENKKCSCVMCFPKGVP RSQEARTESSQASDMMDTMDVENNSTLEKHSGK RRKKRRHRSKVNGLQRGRKKDRPRKHLTLNNKV QKKRWQQRGRKANTRPLKRRRKRGPRIPKDENI NFKQSELPVTCGEVKGTLYKERFKQGTSKKCIQSE DKKWFTPREFEIEGDRGASKNWKLSIRCGGYTLK VLMENKFLPEPPSTRKKRILESHNNTLVDPCEEHK KKNPDASVKFSEFLKKCSETWKTIFAKEKGKFED MAKADKAHYEREMKTYIPPKGEKKKKFKDPNAP KRPPLAFFLFCSEYRPKIKGEHPGLSIDDVVKKLAG MWNNTAAADKQFYEKKAAKLKEKYKKDIAAY RAKGKPNSAKKRVVKAEKSKKKKEEEEDEEDE QEEENEEDDDK
Applications:
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
Formulation:
Liquid at 2.97 mg/mL in storage buffer (50mM Tris, 150mM NaCl, 400mM L- Arginine, 2mM Reduced GSH, 0.2mM Oxidized GSSG, Protease inhibitor, pH 7.4).
Storage:
Store at –80°C. Avoid freezing/thawing cycles.
Quality Control Test:
repeated
BCA to determine quantity of the protein. SDS PAGE to determine purity of the protein
SDS-PAGE Gel
