Human Soluble Liver Antigen / Liver Pancreas Antigen
(Cat. No.: 41A152-s10)
| Origin: | Recombinant | Cat. No.: | 41A152-s10 |
| Concentration: | 1.04 mg/mL | Size: | 1 mg |
| Source: | Spodopterafrugiperda Sf9 | Purity: | >90% |
| Other names: | SLA/LP, SEPSECS | Species: | Human |
Introduction:
SLA/LP catalyzes the last step of sec synthesis by way of converting O- phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) using selenophosphate as the selenium donor. Furthermore, it is considered as a specific marker of autoimmune hepatitis.
Description:
Expressed in insect Sf9 cells with total 525 AA. Mw:58.53KDa(calculated). N- terminal 6xHis-tag and TEV cleavage site, extra 25 AA (highlighted). Recombinant antigen for research use or manufacturing only.
Immunological Function:
As an autoantigen, SLA/LP binds with IgG-type human auto-antibodies.
Amino Acid Sequence:
MSYYHHHHHHDYDIPTTENLYFQGANRES FAAGERLVSPAYVRQGCEARRSHEHLIRLL LEKGKCPENGWDESTLELFLHELAIMDSN NFLGNCGVGEREGRVASALVARRHYRFIHG IGRSGDISAVQPKAAGSSLLNKITNSLVLDII KLAGVHTVANCFVVPMATGMSLTLCFLTL RHKRPKAKYIIWPRIDQKSCFKSMITAGFEP VVIENVLEGDELRTDLKAVEAKVQELGPDC ILCIHSTTSCFAPRVPDRLEELAVICANYDIP HIVNNAYGVQSSKCMHLIQQGARVGRIDAF VQSLDKNFMVPVGGAIIAGFNDSFIQEISKM YPGRASASPSLDVLITLLSLGSNGYKKLLKE RKEMFSYLSNQIKKLSEAYNERLLHTPHNP ISLAMTLKTLDEHRDKAVTQLGSMLFTRQ VSGARVVPLGSMQTVSGYTFRGFMSHTNN YPCAYLNAASAIGMKMQDVDLFIKRLDRCL KAVRKERSKESDDNYDKTEDVDIEEMALK LDNVLLDTYQDASS
Applications:
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
Formulation:
Liquid at 1.04 mg/mL in storage buffer (20mM Tris, 300mM NaCl, 10% Glycerol, pH8.0).
Storage:
Store at –80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test:
BCA to determine quantity of the protein. SDS PAGE to determine purity of the protein.
SDS-PAGE Gel
