Human 2-oxoglutarate Dehydrogenase Complex E2
(Cat. No.: 41A072-s10)
| Origin: | Recombinant | Cat. No.: | 41A072-s10 |
| Concentration: | 0.13 mg/mL | Size: | 1 mg |
| Source: | Spodopterafrugiperda Sf9 | Purity: | >90% |
| Other names: | OGDC-E2, DLST | Species: | Human |
Introduction:
OGDC-E2 is the central E2 component of 2- oxoglutarate dehydrogenase complex (OGDC), which is a mitochondrial multienzyme complex involved in the maintenance of the cellular redox state.
Patients with primary biliary cirrhosis show autoantibodies against the E2 component of 2-oxoglutarate dehydrogenase complex.
Description:
Expressed in insect Sf9 cells with total 411 AA. Mw:45.98KDa(calculated). N-terminal 6xHis-tag and TEV cleavage site, extra 25 AA (highlighted). Recombinant antigen for research use or manufacturing only.
Immunological Function:
As an autoantigen, OGDC-E2 binds with IgG-type human auto-antibodies.
Amino Acid Sequence:
MSYYHHHHHHDYDIPTTENLYFQGAD DLVTVKTPAFAESVTEGDVRWEKAVG DTVAEDEVVCEIETDKTSVQVPSPANG VIEALLVPDGGKVEGGTPLFTLRKTGAA PAKAKPAEAPAAAAPKAEPTAAAVPPP AAPIPTQMPPVPSPSQPPSGKPVSAVKP TVAPPLAEPGAGKGLRSEHREKMNRM RQRIAQRLKEAQNTCAMLTTFNEIDMS NIQEMRARHKEAFLKKHNLKLGFMSAF VKASAFALQEQPVVNAVIDDTTKEVVY RDYIDISVAVATPRGLVVPVIRNVEAMN FADIERTITELGEKARKNELAIEDMDGG TFTISNGGVFGSLFGTPIINPPQSAILGM HGIFDRPVAIGGKVEVRPMMYVALTYD HRLIDGREAVTFLRKIKAAVEDPRVLLL DL
Applications:
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
Formulation:
Liquid at 0.13 mg/mL instorage buffer (20mM Tris, 300mM NaCl, 10% Glycerol, pH8.0).
Storage:
Store at –80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test:
BCA to determine quantity of the protein. SDS PAGE to determine purity of the protein.
SDS-PAGE Gel
