Human Native Histones

(Cat. No.: 41A035)

Introduction:

Histones are proteins that package DNA into nucleosomes and are responsible for maintaining the shape and structure of a nucleosome.
There are five families of histones known to date, H1/H5, H2A, H2B, H3, and H4.
H2A, along with H2B, H3 and H4, is considered as a core histone.

Anti-histone autoantibodies are found in 50%-70% of patients with systemic lupus erythematosus (SLE) and in more than 95% of patients with drug-induced lupus erythematosus.

Description:

Native histones separated from HEK293 cells, including H1, H2A, H2B, H3 and H4. Antigen for research use or manufacturing only.

Immunological Function:

As an autoantigen, histones bind with IgG-type human auto-antibodies.

Applications:

Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

Formulation:

Liquid in storage buffer (50mM H2SO4, 500mM Tris, Protease inhibitor).

Storage:

Store at –80°C. Avoid repeated freezing/thawing cycles.

Quality Control Test:

BCA to determine quantity of the protein. SDS PAGE to determine purity of the protein.

SDS-PAGE Gel