Human Glutamic Acid Decarboxylase 65kDa Isoform (Full-length GAD65)
(Cat. No.: 41842)
| Origin: | Recombinant | Cat. No.: | 41842 |
| Tag: | N-terminal 6xHis | Size: | 0.1 mg |
| Source: | Spodoptera frugiperda Sf9 | Purity: | >95% |
| Other Names: | fl-GAD65 | Species: | Human |
Introduction:
GAD65 is primarily expressed in neuron cells and pancreas 𝜷-cells. It works as a catalyser in GABA synthesis.
Glutamic acid decarboxylase autoantibodies (GADA) are found in 70% to 80% of individuals with new-onset type 1 diabetes, making it the most frequent autoantibody in autoimmune diabetes. GADA can be detected in serum for many years post diagnosis, and high concentrations of GADA have been considered as a marker of faster 𝜷-cell exhaustion in these patients. Furthermore, GADA in nondiabetic individuals predicts the later development of type 1 diabetes1. Besides autoimmune diabetes, GADA also exists in Stiff Man Syndrome, autoimmune polyendocrinopathies, and some of Grave’s Disease patients.
Description:
Expressed in Baculovirus-sf9 vector expression system with total 612 amino acids (AA).Mw: 67.65 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted). Recombinant antigen for research use or manufacturing only.
Immunological Function:
As an autoantigen, GAD65 binds with IgG- type human autoantibodies.
Applications:
Bio-functional study, ELISA, radioimmunoassay
Amino Acid Sequence:
MSYYHHHHHHDYDIPTTENLYFQGAASPGSGF WSFGSEDGSGDSENPGTARAWCQVAQKFTGGIGN KLCALLYGDAEKPAESGGSQPPRAAARKAACACD QKPCSCSKVDVNYAFLHATDLLPACDGERPTLAFL QDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYN
WELADQPQNLEEILMHCQTTLKYAIKTGHPRYFN QLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFV LLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMY AMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHF SLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRIL EAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICK KYKIWMHVDAAWGGGLLMSRKHKWKLSGVERA NSVTWNPHKMMGVPLQCSALLVREEGLMQNCN QMHASYLFQQDKHYDLSYDTGDKALQCGRHVDV FKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIK NREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNE ERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKV NFFRMVISNPAATHQDIDFLIEEIERLGQDLSR
Formulation:
Liquid in Phosphate buffer containing NaCl (1M), Na2HPO4 (10mM), KCl (2.7mM) and KH2PO4 (2.0mM) with protease inhibitor, pH8.0.
Storage:
Store at –80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test:
BCA to determine quantity of the protein. SDS PAGE to determine purity of the protein. Elisa assay analysis to determine functionality of protein.
SDS-PAGE Gel
