Histone H2B

Histones are proteins that package DNA into nucleosomes and are responsible for maintaining the shape and structure of a nucleosome.

There are five families of histones known to date, H1/H5, H2A, H2B, H3, and H4. H2B, along with H2A, H3 and H4, is considered as a core histone.

Anti-histone autoantibodies are found in 50%-70% of patients with systemic lupus erythematosus (SLE) and in more than 95% of patients with drug-induced lupus erythematosus.

As an autoantigen, H2B binds with IgG-type human auto-antibodies.

METSYYHHHH HHDYDIPTTE NLYFQGAPEP AKSAPAPKKG SKKAVTKAQK KDGKKRKRSR
KESYSIYVYK VLKQVHPDTG ISSKAMETGI METNSFVNDI FERIAGEASR LAHYNKRSTI
TSREIQTAVR LLLPGELAKH AVSEGTKAVT KYTSSK

Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

Lyophilized at 1 mg/mL in storage buffer (20mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolve completely.

Expressed in E.coli cells with total 156 AA. Mw: 17.57 KDa (calculated).

N-terminal 6xHis-tag and TEV cleavage site, 27 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.  Avoid repeatedfreezing /thawing cycles.

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.