Scl-70 (DNA Topoisomerase I)
| Origin: | Recombinant | Cat. No.: | 41630 |
| Tag: | N-terminal 6xHis | Size: | 0.1 mg |
| Source: | Spodoptera frugiperda Sf9 | Purity: | >90% |
| Other Names: | TOP1 | Species: | Human |
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INTRODUCTION
Type I topoisomerase, also called as TOP1 and Scl-70, is an enzyme responsible for cutting one of the two strands of double-stranded DNA, which interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. Scl-70 is a target antigen for anti-Scl-70 autoantibody, which is a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis.
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IMMUNOLOGICAL FUNCTION
As an autoantigen, Scl-70 binds with IgG-type human auto-antibodies.
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AMINO ACID SEQUENCE
MSYYHHHHHHDYDIPTTENLYFQGASGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRKEEKVRASGDAKIKKEKENGFSSPPQIKDEPEDDGYFVPPKEDIKPLKRPRDEDDADYKPKKIKTEDTKKEKKRKLEEEEDGKLKKPKNKDKDKKVPEPDNKKKKPKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF
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APPLICATIONS
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
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FORMULATION
Lyophilized at 1 mg/mL in storage buffer (20mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).
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RECONSTITUTION
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolve completely.
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DESCRIPTION
Expressed in insect Sf9 cells with total 789 AA. Mw: 93.7 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
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STORAGE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeatedfreezing /thawing cycles.
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QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
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SDS-PAGE Gel
