CENPB (Centromere Protein B)
| Origin: | Recombinant | Cat. No.: | 41620 |
| Tag: | N-terminal 6xHis | Size: | 0.1 mg |
| Source: | Spodoptera frugiperda Sf9 | Purity: | >90% |
| Other Names: | CENPB | Species: | Human |
INTRODUCTION
Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. The centromere protein B (CENPB) is one of them. CENPB is a DNA-binding protein derived from transposases of the pogo DNA transposon family and plays an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. CENPB is the most important centromeric autoantigens and auantibodies against CENPB are present in the sera of up to 80% of patients with CREST syndrome.
IMMUNOLOGICAL FUNCTION
As an autoantigen, CENP-B binds with IgG-type human auto-antibodies.
AMINO ACID SEQUENCE
MSYYHHHHHHDYDIPTTENLYFQGAGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNKLSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRHGVVSCSGVARARARNAAPRTPAAPASPAAVPSEGSGGSTTGWRAREEQPPSVAEGYASQDVFSATETSLWYDFLPDQAAGLCGGDGRPRQATQRLSVLLCANADGSEKLPPLVAGKSAKPRAGQAGLPCDYTANSKGGVTTQALAKYLKALDTRMAAESRRVLLLAGRLAAQSLDTSGLRHVQLAFFPPGTVHPLERGVVQQVKGHYRQAMLLKAMAALEGQDPSGLQLGLTEALHFVAAAWQAVEPSDIAACFREAGFGGGPNATITTSLKSEGEEEEEEEEEEEEEEGEGEEEEEEGEEEEEEGGEGEELGEEEEVEEEGDVDSDEEEEEDEESSSEGLEAEDWAQGVVEAGGSFGAYGAQEEAQCPTLHFLEGGEDSDSDSEEEDDEEEDDEDEDDDDDEEDGDEVPVPSFGEAMAYFAMVKRYLTSFPIDDRVQSHILHLEHDLVHVTRKNHARQAGVRGLGHQS
APPLICATIONS
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
FORMULATION
Lyophilized at 1 mg/mL in storage buffer (20mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).
RECONSTITUTION
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolve completely.
DESCRIPTION
Expressed in insect Sf9 cells with total 623 AA. Mw: 68.1 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
STORAGE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeatedfreezing /thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
