La/SSB (Human Sjogren Syndrome Antigen B)
Human Sjogren Syndrome Antigen B (La/SSB)- 41600
| Origin: | Recombinant | Cat. No.: | 41600-s10 |
| Tag: | N-terminal 6xHis | Size: | 1 mg |
| Source: | Spodoptera frugiperda Sf9 | Purity: | >90% |
| Other names: | La/SSB | Species: | Human |
-
INTRODUCTION
La/SSB is a nuclear phosphoprotein involved in various aspects of RNA metabolisms. It can bind with transcripts of RNA polymerase III, process pre-tRNA precursors and act as an RNA chaperone. Autoantibody against La/SSB are most frequently found in Sjogren’s Syndrome, Systemic Lupus Erythematosus (SLE) and Subacute Cutaneous Lupus.
-
IMMUNOLOGICAL FUNCTION
As an autoantigen, SSB/La binds with IgG-type human auto-antibodies.
-
AMINO ACID SEQUENCE
MSYYHHHHHHDYDIPTTENLYFQGAAENGDNEKMAALEAKICHQIEYYFGDFNLPRDKFLKEQIKLDEGWVPLEIMIKFNRLNRLTTDFNVIVEALSKSKAELMEISEDKTKIRRSPSKPLPEVTDEYKNDVKNRSVYIKGFPTDATLDDIKEWLEDKGQVLNIQMRRTLHKAFKGSIFVVFDSIESAKKFVETPGQKYKETDLLILFKDDYFAKKNEERKQNKVEAKLRAKQEQEAKQKLEEDAEMKSLEEKIGCLLKFSGDLDDQTCREDLHILFSNHGEIKWIDFVRGAKEGIILFKEKAKEALGKAKDANNGNLQLRNKEVTWEVLEGEVEKEALKKIIEDQQESLNKWKSKGRRFKGKGKGNKAAQPGSGKGKVQFQGKKTKFASDDEHDEHDENGATGPVKRAREETDKEEPASKQQKTENGAGDQ
-
APPLICATIONS
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
-
FORMULATION
Lyophilized at 1 mg/mL in storage buffer (20mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).
-
RECONSTITUTION
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
-
DESCRIPTION
Expressed in insect Sf9 cells with total 432 AA. Mw: 49.8 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
-
STORAGE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeatedfreezing /thawing cycles.
-
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
-
SDS-PAGE Gel
