U-snRNP B/B’
Human Small Ribonucleoprotein Associated Protein B B’ (SmB/B’)
| Origin: | Recombinant | Cat. No.: | 41530-s1 |
| Tag: | N-terminal 6xHis | Size: | 1 mg |
| Source: | Spodoptera frugiperda Sf9 | Purity: | >90% |
| Other Names: | SmB/B’, snRNPB/B’ | Species: | Human |
INTRODUCTION
Small nuclear ribonucleoprotein complexes (abbreviated as U-snRNP) are essential for splicing of precursor mRNA molecules and snRNPB is one of several nuclear proteins which are found in common among U1, U2, U4/U6, and U5 small ribonucleoprotein particles (snRNPs). Autoantibodies from patients with systemic lupus erythematosus (SLE) recognize epitopes on snRNPB/B’.
IMMUNOLOGICAL FUNCTION
As an autoantigen, SmB/B’ binds with IgG-type human auto-antibodies.
AMINO ACID SEQUENCE
MSYYHHHHHHDYDIPTTENLYFQGATVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGIGRAAGRGIPAGVPMPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAAAATASIAGAPTQYPPGRGGPPPPMGRGAPPPGMMGPPPGMRPPMGPPMGIPPGRGTPMGMPPPGMRPPPPGMRGPPPPGMRPPRP.
APPLICATIONS
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
FORMULATION
Lyophilized at 1 mg/mL in storage buffer (20mM Tris, 300-500mM NaCl, 10% Glycerol, PH8.0).
RECONSTITUTION
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let thelyophilized pellet dissolve completely.
DESCRIPTION
Expressed in insect Sf9 cells with total 265 AA. Mw: 27.6 KDa (calculated).
N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only
STORAGE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeatedfreezing /thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
SDS-PAGE Gel
