Human U1 Small Nuclear Ribonucleoprotein particle 68/70 (U1- snRNP68/70)

(Cat. No.: 41500-s10)

Introduction:

Small nuclear ribonucleoprotein complexes are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small uridylate-rich RNA (U1 RNA) complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1- snRNP particle, whereas 7 so-called Sm proteins (B/B’, D1, D2, D3, E, F, G) form a core subparticle that is common to all U- snRNP complexes.

Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients.

Description:

Expressed in E.coli with total 480 AA. Mw:56.5 kDa(calculated).
N-terminal 6xHis-tag and TEV cleavage site, 44 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

Immunological Function:

As an autoantigen, RNP68/70 binds with IgG-type human auto-antibodies.

Applications:

Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

Amino Acid Sequence:

MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSENLYFQGATQFLPPNLLALFAPRDPIPYLPPLEKLPHEKHHNQPYCGIAPYIREFEDPRDAPPPTRAETREERMERKRREKIERRQQEVETELKMWDPHNDPNAQGDAFKTLFVARVNYDTTESKLRREFEVYGPIKRIHMVYSKRSGKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIRHSGRDDTSRYDERPGPSPLPHRDRDRDRERERRERSRERDKERERRRSRSRDRRRRSRSRDKEERRRSRERSKDKDRDRKRRSSRSRERARRERERKEELRGGGGDMAEPSEAGDAPPDDGPPGELGPDGPDGPEEKGRDRDRERRRSHRSERERRRDRDRDRDRDREHKRGERGSERGRDEARGGGGGQDNGLEGLGNDSRDMYMESEGGDGYLAPENGYLMEAAPE

Formulation:

Liquid at 2.44 mg/mL in 8M Urea buffer with protease inhibitor.

Storage:

–80°C. Avoid

repeated

Store at freezing/thawing cycles.

Quality Control Test:

BCA to determine quantity of the protein. SDS PAGE to determine purity of the protein. Immunodot analysis to determine functionality of protein.

SDS-PAGE Gel

Dot Blot Assay