NTRODUCTION
Insulin is a hormone synthesized by the b cells of pancreatic islets. It consists of two amino acid chains, A chain and B chain, linked with a sulphide bond. The A chain is made of 21 amino acids and B chain with 30 amino acids. Insulin has a molecular weight of 5.8KDa1.
ASSAY CHARACTERISTICS
A. Sensitivity
The lowest insulin level that can be measured by this assay is 0.025 ng/ml.
B. Precision
Intra-assay Precision (Precision within an assay) C.V. < 10%.
Inter-assay Precision (Precision between assays) C.V. <10%.
C. Recovery
The recovery of the assay was determined by adding various amounts insulin to a sample. The measured concentration of the spiked sample in the assay was compared to the expected concentration. The average recovery was 92%.
D. Specificity: Will update soon
E. Sample : Serum and plasma
F. Storage: 4°C
References:
1- Mane K et al.,(2012) J Basic Clin Pharm 3(2):283-293
