Human X-ray Repair Cross-complementing Protein 5/6 (Ku70/80)
(Cat. No.: 41A180-s10)
| Origin: | Recombinant | Cat. No.: | 41A180-s10 |
| Tag: | N-terminal 6xHis | Size: | 1 mg |
| Source: | Spodoptera frugiperda Sf9 | Purity: | >90% |
| Other Names: | Ku70/80, Ku70 80, Ku | Species: | Human |
Introduction:
The Ku autoantigen is a heterodimer of 70kDa (p70) and 80kDa (p80) proteins. AntiKu antibodies are reported in various connective tissue diseases and the Ku complex can be responsible for a very strong autoimmune answer in autoimmune disease.
Description:
Heterodimer expressed in insect Sf9 cells containing a 633 AA (Mw: 72.8 KDa) Ku70 and a 743 AA (Mw: 84.1 KDa) Ku80.
Ku70 has N-terminal 6xHis-tag and TEV cleavage site with 25 extra AA (highlighted), while Ku80 has C-terminal Flag-tag with 21 extra AA (highlighted). Recombinant antigen for research use or manufacturing only.
Immunological Function:
As an autoantigen, Ku70/80 binds with IgG-type human auto-antibodies.
Amino Acid Sequence:
Ku70: MSYYHHHHHHDYDIPTTENLYFQGASGWESYYKTEGDEEAE EEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFD MSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQE LDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWV CANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAG DLRDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEES SKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKA LKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSR QIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVY PEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVP QEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQ VGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPE QAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRK HDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAY GLKSGLKKQELLEALTKHFQD
Ku80:
MVRSGNKAAVVLCMDVGFTMSNSIPGIESPFEQAKKVITMFVQ RQVFAENKDEIALVLFGTDGTDNPLSGGDQYQNITVHRHLMLP DFDLLEDIESKIQPGSQQADFLDALIVSMDVIQHETIGKKFEKRH IEIFTDLSSRFSKSQLDIIIHSLKKCDISLQFFLPFSLGKEDGSGDR GDGPFRLGGHGPSFPLKGITEQQKEGLEIVKMVMISLEGEDGLD EIYSFSESLRKLCVFKKIERHSIHWPCRLTIGSNLSIRIAAYKSILQ ERVKKTWTVVDAKTLKKEDIQKETVYCLNDDDETEVLKEDIIQ GFRYGSDIVPFSKVDEEQMKYKSEGKCFSVLGFCKSSQVQRRFF MGNQVLKVFAARDDEAAAVALSSLIHALDDLDMVAIVRYAYDK RANPQVGVAFPHIKHNYECLVYVQLPFMEDLRQYMFSSLKNSK KYAPTEAQLNAVDALIDSMSLAKKDEKTDTLEDLFPTTKIPNP RFQRLFQCLLHRALHPREPLPPIQQHIWNMLNPPAEVTTKSQI PLSKIKTLFPLIEAKKKDQVTAQEIFQDNHEDGPTAKKLKTEQG GAHFSVSSLAEGSVTSVGSVNPAENFRVLVKQKKASFEEASNQL INHIEQFLDTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKA LQEKVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEEAKKFL APKDKPSGDTAAVFEEGGDVDDLLDMISYYDYKDDDDK
Applications:
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.
Formulation:
Liquid in storage buffer (50mM Tris, 300- 500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).
Storage:
Store at –80°C. Avoid repeated freezing /thawing cycles.
Quality Control Test:
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein. Immunodot analysis to determine functionality of protein.
SDS-PAGE Gel
